The buckwheat (Fagopyrum esculentum) is very healthy for human nutrition with its rich protein, carbohydrate, mineral and vitamin content. It is also among the functional foods that reduce the risk of disease especially used by celiac patients. The buckwheat plant needs an advanced root system for its healthy growth. It has been found that there is a very few studies in the literature on root morphological characteristics of buckwheat plant. The aim of study is to investigate the effect of organic and inorganic fertilizer applications on two different buckwheat varieties some plant root parameters (length, area, volume and diameter). The experiment was established as randomize design in split-plots in 2019 in Cukurova University, Department of Field Crops Research and Application. Aktaş and Güneş buckwheat varieties were used in the experiment. Five different fertilizers (Control, Urea, Worm-manure, Chickenmanure, Cattle-manure) were used. Buckwheat cultivar were cultivated in April 2019 and harvested in July 2019. After harvesting, the roots were sampled, washed and the morphological characteristics were determined by WinRhizo program. In terms of root length, statistically a significant difference was found between the fertilizer treatments. In terms of root length, the best practice was chickenmanure (39.7 km.da-1), followed by mineral urea (27.8 km.da-1). Statistically significant differences were determined between different buckwheat varieties and different fertilizers in terms of root area and root volume parameters. Aktaş cultivar increased the root growth better than that of Güneş cultivar. Among the fertilizer applications, chicken-manure and urea fertilizer applications increased root growth better than control treatment.