This research was carried out to determine the effect of zinc (Zn) application by soil on yield and seed oil content of two winter canola (Brassica napus ssp. oleifera L.) cultivars (cv.Samourai and cv. Zorro) in two experimental years. The experimental design of the study was a randomized complete block with four replications. 3 kg da-1 Zinc was applied as zinc sulphate (ZnSO4.7H2O) fertilizer to the soil before sowing. According to the results of the study examined in traits such as plant height, number of lateral branches, number of capsule per plant, capsule length, number of seed per capsule, seed yield and seed oil content; seed yield was affected to be increased significantly, oil percent showed a tendency to be increased at insignificant level statistically by the Zn application. Cultivars showed variations in plant height, the number of lateral branches and thousand seed weight. Cultivars were affected differently by the zinc application. Samourai has the best response to Zn application for increase in seed yield, and the data varied according to years.