This study was caaried out to determine the effects of surface drip (SDI) and subsurface drip irrigation (SSDI) and different amount of irrigation water on soil temperature and biomass during 2016 and 2017 years in Diyarbakır Province of Southeast Anatolia Region in Turkey. The experimental design was split plots in ramdomized bloks with three replications. The main plots had SDI and SSDI with sub-plots in different rates of real-time crop evapotranspiration based on FAO-56 Penman-Monteith (FAO-56 PM) as different amount of irrigation water. The treatments: main plots:: I1 : SDI, I2: SSDI-30 cm, I3: SSDI-40 cm; Subplots: K1 : I=ETc x 1.25, K2 : I=ETc x 1.0 ve K3: I=ETcx0.75. Irrigation interval was 5 days. According to the temperature measurements before irrigation at 10 cm near of the lateral for different depths, the temperature was higher 1.47, 1.53, 1.25 and 0.69 OC at 35 cm, 30 cm, 20 cm and 10 cm of depths for the SDI than those for SSDI, respectively. This results attributed that the upper layers of the soil are more wetted by the SDI than SSDI, thus this zone of soil occured more cooler. In adition, considering the depth of 40 cm for SSDI and moving of some water to the down, upper layer could be cooler compared to the lower layers of soil. Thus, plant root development and biomass was higher under the SSDI conditions. The maximum biomass (8.79 t ha-1) and lint yield (1865 kg ha-1) was obtained from the treatment in which the SSDI with the depth of 40 cm and amount of irrigation applied under the the rate of 1.25 of crop evapotranspiration.